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A novel assembly mechanism for the DNA polymerase III holoenzyme DnaX complex: association of δδ′ with DnaX(4) forms DnaX(3)δδ′
We have constructed a plasmid-borne artificial operon that expresses the six subunits of the DnaX complex of Escherichia coli DNA polymerase III holoenzyme: τ, γ, δ, δ′, χ and ψ. Induction of this operon followed by assembly in vivo produced two τγ mixed DnaX complexes with stoichiometries of τ(1)γ(...
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| Gepubliceerd in: | EMBO J |
|---|---|
| Hoofdauteurs: | , , , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
Nature Publishing Group
2000
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC305859/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11101526/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/19.23.6536 |
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