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Efficient in Vivo Manipulation of Alternative Pre-mRNA Splicing Events Using Antisense Morpholinos in Mice

Mammalian pre-mRNA alternative splicing mechanisms are typically studied using artificial minigenes in cultured cells, conditions that may not accurately reflect the physiological context of either the pre-mRNA or the splicing machinery. Here, we describe a strategy to investigate splicing of normal...

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Bibliografiska uppgifter
Huvudupphovsmän: Parra, Marilyn K., Gee, Sherry, Mohandas, Narla, Conboy, John G.
Materialtyp: Artigo
Språk:Inglês
Publicerad: American Society for Biochemistry and Molecular Biology 2011
Ämnen:
RNA
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3057813/
https://ncbi.nlm.nih.gov/pubmed/21156798
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.158154
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