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The BH1999 Protein of Bacillus halodurans C-125 Is Gentisyl-Coenzyme A Thioesterase

In this study, we have shown that recombinant BH1999 from Bacillus halodurans catalyzes the hydrolysis of gentisyl coenzyme A (CoA) (2,5-dihydroxybenzoyl-coenzyme A) at physiological pH with a k(cat)/K(m) of 1.6 × 10(6) M(−1) s(−1) and the hydrolysis of 3-hydroxybenzoyl-CoA with a k(cat)/K(m) of 3.0...

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Detalhes bibliográficos
Main Authors: Zhuang, Zhihao, Song, Feng, Takami, Hideto, Dunaway-Mariano, Debra
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC305745/
https://ncbi.nlm.nih.gov/pubmed/14702308
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.186.2.393-399.2004
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