Simultaneous Detection of Single Molecules and Singulated Ensembles of Molecules Enables Immunoassays with Broad Dynamic Range

We report a method for combining the detection of single molecules (digital) and an ensemble of molecules (analog) that is capable of detecting enzyme label from 10(−19) M to 10(−13) M, for use in high sensitivity enzyme-linked immunosorbent assays (ELISA). The approach works by capturing proteins o...

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Detalhes bibliográficos
Main Authors: Rissin, David M., Fournier, David R., Piech, Tomasz, Kan, Cheuk W., Campbell, Todd G., Song, Linan, Chang, Lei, Rivnak, Andrew J., Patel, Purvish P., Provuncher, Gail K., Ferrell, Evan P., Howes, Stuart C., Pink, Brian A., Minnehan, Kaitlin A., Wilson, David H., Duffy, David C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3056883/
https://ncbi.nlm.nih.gov/pubmed/21344864
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac103161b
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