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PI 3-Kinase/Rac1 and ERK1/2 Regulate FGF-2–Mediated Cell Proliferation through Phosphorylation of p27 at Ser10 by KIS and at Thr187 by Cdc25A/Cdk2

PURPOSE. To determine the mechanism of p27 phosphorylation through common and differential pathways triggered by FGF-2 in corneal endothelial cells (CECs). METHODS. A GTP pull-down assay was performed to identify Rac1-GTP. Expression and activation of protein were analyzed by immunoblotting. Cell pr...

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Detalhes bibliográficos
Main Authors: Lee, Jeong Goo, Kay, EunDuck P.
Formato: Artigo
Idioma:Inglês
Publicado em: Association for Research in Vision and Ophthalmology, Inc. 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3053288/
https://ncbi.nlm.nih.gov/pubmed/20811053
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1167/iovs.10-6140
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