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Expression, purification, crystallization and preliminary X-ray analysis of the DNA-binding domain of Rhodobacter capsulatus MopB

The LysR-type regulator MopB represses transcription of several target genes (including the nitrogen-fixation gene anfA) in Rhodobacter capsulatus at high molybdenum concentrations. In this study, the isolated DNA-binding domain of MopB (MopB(HTH)) was overexpressed in Escherichia coli. Purified Mop...

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Detalhes bibliográficos
Main Authors: Müller, Alexandra, Schlicker, Christine, Fehringer, Maria, Masepohl, Bernd, Hofmann, Eckhard
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3053167/
https://ncbi.nlm.nih.gov/pubmed/21393847
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110054710
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