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Multiple point mutations in a shuttle vector propagated in human cells: evidence for an error-prone DNA polymerase activity.

Mutagenesis was studied at the DNA-sequence level in human fibroblast and lymphoid cells by use of a shuttle vector plasmid, pZ189, containing a suppressor tRNA marker gene. In a series of experiments, 62 plasmids were recovered that had two to six base substitutions in the 160-base-pair marker gene...

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Detalhes bibliográficos
Main Authors: Seidman, M M, Bredberg, A, Seetharam, S, Kraemer, K H
Formato: Artigo
Idioma:Inglês
Publicado em: 1987
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC305223/
https://ncbi.nlm.nih.gov/pubmed/3474635
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