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Multiple point mutations in a shuttle vector propagated in human cells: evidence for an error-prone DNA polymerase activity.
Mutagenesis was studied at the DNA-sequence level in human fibroblast and lymphoid cells by use of a shuttle vector plasmid, pZ189, containing a suppressor tRNA marker gene. In a series of experiments, 62 plasmids were recovered that had two to six base substitutions in the 160-base-pair marker gene...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1987
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC305223/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3474635/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.14.4944 |
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