Expression of the alpha-bungarotoxin binding site of the nicotinic acetylcholine receptor by Escherichia coli transformants.
Restriction fragments of DNA derived from a cDNA clone of the alpha subunit of the acetylcholine receptor were subcloned in Escherichia coli by using the trpE fusion vector, pATH2. Transformants expressing the amino acid sequences 166-315 or 166-200 are shown to produce a chimeric protein that bound...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteur principal: | |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1987
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC305076/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3295881/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.12.4318 |
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