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RecA K72R Filament Formation Defects Reveal an Oligomeric RecA Species Involved in Filament Extension
Using an ensemble approach, we demonstrate that an oligomeric RecA species is required for the extension phase of RecA filament formation. The RecA K72R mutant protein can bind but not hydrolyze ATP or dATP. When mixed with other RecA variants, RecA K72R causes a drop in the rate of ATP hydrolysis a...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Biochemistry and Molecular Biology
2011
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3048670/ https://ncbi.nlm.nih.gov/pubmed/21193798 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.194407 |
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