Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons

Bacterial diversity among environmental samples is commonly assessed with PCR-amplified 16S rRNA gene (16S) sequences. Perceived diversity, however, can be influenced by sample preparation, primer selection, and formation of chimeric 16S amplification products. Chimeras are hybrid products between m...

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Main Authors: Haas, Brian J., Gevers, Dirk, Earl, Ashlee M., Feldgarden, Mike, Ward, Doyle V., Giannoukos, Georgia, Ciulla, Dawn, Tabbaa, Diana, Highlander, Sarah K., Sodergren, Erica, Methé, Barbara, DeSantis, Todd Z., Petrosino, Joseph F., Knight, Rob, Birren, Bruce W.
格式: Artigo
語言:Inglês
出版: Cold Spring Harbor Laboratory Press 2011
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3044863/
https://ncbi.nlm.nih.gov/pubmed/21212162
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.112730.110
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