Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome

AIM: To design and validate broad-range 16S rRNA primers for use in high throughput sequencing to classify bacteria isolated from the human foregut microbiome. METHODS: A foregut microbiome dataset was constructed using 16S rRNA gene sequences obtained from oral, esophageal, and gastric microbiomes...

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Detalhes bibliográficos
Main Authors: Nossa, Carlos W, Oberdorf, William E, Yang, Liying, Aas, Jørn A, Paster, Bruce J, DeSantis, Todd Z, Brodie, Eoin L, Malamud, Daniel, Poles, Michael A, Pei, Zhiheng
Formato: Artigo
Idioma:Inglês
Publicado em: Baishideng Publishing Group Co., Limited 2010
Assuntos:
Original Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2932916/
https://ncbi.nlm.nih.gov/pubmed/20806429
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v16.i33.4135
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