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A 96-well plate assay for high-throughput analysis of holocarboxylase synthetase activity

BACKGROUND: Holocarboxylase synthetase (HCS) catalyzes the covalent binding of biotin to both carboxylases and histones. Biotinylated carboxylases and biotinylated histones play crucial roles in the metabolism of fatty acids, amino acids, and glucose, and in gene regulation and genome stability, res...

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Main Authors: Rios-Avila, Luisa, Prince, Sara A., Wijeratne, Subhashinee S. K., Zempleni, Janos
Formato: Artigo
Idioma:Inglês
Publicado: 2010
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3043159/
https://ncbi.nlm.nih.gov/pubmed/21195703
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cca.2010.12.031
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