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Escherichia coli formylmethionine tRNA: mutations in GGGCCC sequence conserved in anticodon stem of initiator tRNAs affect initiation of protein synthesis and conformation of anticodon loop.

We have generated mutants of Escherichia coli formylmethionine initiator tRNA in which one, two, and all three G X C base pairs in the GGGCCC sequence in the anticodon stem are changed to those found in E. coli elongator methionine tRNA. Overproduction of the mutant tRNAs using M13 recombinants as a...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Seong, B L, RajBhandary, U L
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1987
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC304201/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3540960/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.2.334
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