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Optimization of PCR conditions to amplify microsatellite loci in the bunchgrass lizard (Sceloporus slevini) genomic DNA

BACKGROUND: Microsatellites, also called Simple Sequence Repeats (SSRs), repetitions of nucleotide motifs of 1-5 bases, are currently the markers of choice due to their abundant distribution in the genomes, and suitability for high-throughput analysis. A total of five different primer pairs were opt...

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Detalhes bibliográficos
Main Authors: Narina, Satya S, d'Orgeix, Christian A, Sayre, Brian L
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3041990/
https://ncbi.nlm.nih.gov/pubmed/21281503
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-4-26
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