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A quantitative assay for measuring mRNA decapping by splinted ligation reverse transcription polymerase chain reaction: qSL-RT-PCR
The degradation of messenger RNA is a critical node of gene regulation. A major pathway of mRNA decay is initiated by shortening of the poly(A) tail, followed by removal of the 5′ cap structure (decapping) and subsequent degradation. Decapping is an important determinate in the destruction of many t...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Cold Spring Harbor Laboratory Press
2011
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3039152/ https://ncbi.nlm.nih.gov/pubmed/21220548 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2436411 |
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