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Inhibitor Binding Increases the Mechanical Stability of Staphylococcal Nuclease
Staphylococcal nuclease (SNase) catalyzes the hydrolysis of DNA and RNA in a calcium-dependent fashion. We used AFM-based single-molecule force spectroscopy to investigate the mechanical stability of SNase alone and in its complex with an SNase inhibitor, deoxythymidine 3′,5′-bisphosphate. We found...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3037565/ https://ncbi.nlm.nih.gov/pubmed/21320455 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2011.01.011 |
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