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Inhibitor Binding Increases the Mechanical Stability of Staphylococcal Nuclease

Staphylococcal nuclease (SNase) catalyzes the hydrolysis of DNA and RNA in a calcium-dependent fashion. We used AFM-based single-molecule force spectroscopy to investigate the mechanical stability of SNase alone and in its complex with an SNase inhibitor, deoxythymidine 3′,5′-bisphosphate. We found...

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Autors principals: Wang, Chien-Chung, Tsong, Tian-Yow, Hsu, Yau-Heiu, Marszalek, Piotr E.
Format: Artigo
Idioma:Inglês
Publicat: The Biophysical Society 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3037565/
https://ncbi.nlm.nih.gov/pubmed/21320455
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2011.01.011
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