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Determining under- and oversampling of individual particle distributions in microfluidic electrophoresis with orthogonal laser-induced fluorescence detection

This report investigates the effects of sample size on the separation and analysis of individual biological particles using microfluidic devices equipped with an orthogonal LIF detector. A detection limit of 17 ± 1 molecules of fluorophore is obtained using this orthogonal LIF detector under a const...

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Bibliografiske detaljer
Main Authors: Whiting, Christofer E., Dua, Rajat A., Duffy, Ciarán F., Arriaga, Edgar A.
Format: Artigo
Sprog:Inglês
Udgivet: 2008
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3037013/
https://ncbi.nlm.nih.gov/pubmed/18386300
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.200700470
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