Enzymatic assessment of cholesterol on electrophoresis gels for estimating HDL size distribution and plasma concentrations of HDL subclasses

The aim of this study was to develop an enzymatic cholesterol staining method to determine HDL subclasses in a polyacrylamide gradient gel electrophoresis, which further allows staining by protein in the same electrophoresis lane. HDLs from 120 healthy individuals were separated through nondenaturin...

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Detalles Bibliográficos
Autores principales: Toledo-Ibelles, Paola, García-Sánchez, Cynthia, Ávila-Vazzini, Nydia, Carreón-Torres, Elizabeth, Posadas-Romero, Carlos, Vargas-Alarcón, Gilberto, Pérez-Méndez, Oscar
Formato: Artigo
Lenguaje:Inglês
Publicado: The American Society for Biochemistry and Molecular Biology 2010
Materias:
Methods
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3035525/
https://ncbi.nlm.nih.gov/pubmed/20097938
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D004358
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