Enzymatic assessment of cholesterol on electrophoresis gels for estimating HDL size distribution and plasma concentrations of HDL subclasses

The aim of this study was to develop an enzymatic cholesterol staining method to determine HDL subclasses in a polyacrylamide gradient gel electrophoresis, which further allows staining by protein in the same electrophoresis lane. HDLs from 120 healthy individuals were separated through nondenaturin...

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Detalhes bibliográficos
Main Authors: Toledo-Ibelles, Paola, García-Sánchez, Cynthia, Ávila-Vazzini, Nydia, Carreón-Torres, Elizabeth, Posadas-Romero, Carlos, Vargas-Alarcón, Gilberto, Pérez-Méndez, Oscar
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2010
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3035525/
https://ncbi.nlm.nih.gov/pubmed/20097938
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D004358
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