Subcloning and expression of the Brucella abortus L7/L12 ribosomal gene and T-lymphocyte recognition of the recombinant protein.
The Brucella abortus L7/L12 ribosomal gene was amplified by PCR and subcloned into the prokaryotic expression vector pMAL-c2. Escherichia coli DH5 alpha was transformed with the pMAL-L7/L12 construct, and gene expression was induced by IPTG (isopropyl-beta-D-thiogalactopyranoside). The resulting fus...
সংরক্ষণ করুন:
| প্রকাশিত: | Infect Immun |
|---|---|
| প্রধান লেখক: | , |
| বিন্যাস: | Artigo |
| ভাষা: | Inglês |
| প্রকাশিত: |
American Society for Microbiology (ASM)
1994
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| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC303250/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7927808/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.62.11.5201-5204.1994 |
| ট্যাগগুলো: |
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