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Development of a Visual RPA-CRISPR/Cas12a Assay Targeting <i>Bcsp31</i> for Rapid Detection of <i>Brucella</i> spp.

To establish a rapid nucleic acid detection method for field screening of <i>Brucella</i> spp., a visual assay based on recombinase polymerase amplification (RPA) combined with CRISPR/Cas12a was developed. Four pairs of RPA primers and two crRNAs were designed according to the conserved <i>Brucella...

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Hoofdauteurs: Shuairan Zhang, Yu Zhang, Cai Yin, Xiaona Zang, Minjing Jin, Yawen Wu, Taotao Bai, Long Ma, Yong Shi, Zhixin Li, Xiaoliang Wang
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: MDPI AG 2026-08-01
Reeks:Veterinary Sciences
Onderwerpen:
<i>Brucella</i> spp.
brucellosis
bcsp31
recombinase polymerase amplification
CRISPR/Cas12a
visual detection
Online toegang:https://www.mdpi.com/2306-7381/13/8/820
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