Characterizing Ligand-Gated Ion Channel Receptors with Genetically Encoded Ca(++) Sensors

We present a cell based system and experimental approach to characterize agonist and antagonist selectivity for ligand-gated ion channels (LGIC) by developing sensor cells stably expressing a Ca(2+) permeable LGIC and a genetically encoded Förster (or fluorescence) resonance energy transfer (FRET)-b...

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Autors principals: Yamauchi, John G., Nemecz, Ákos, Nguyen, Quoc Thang, Muller, Arnaud, Schroeder, Lee F., Talley, Todd T., Lindstrom, Jon, Kleinfeld, David, Taylor, Palmer
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2011
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Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3030600/
https://ncbi.nlm.nih.gov/pubmed/21305050
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0016519
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