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Accurate quantification of transcriptome from RNA-Seq data by effective length normalization

We propose a novel, efficient and intuitive approach of estimating mRNA abundances from the whole transcriptome shotgun sequencing (RNA-Seq) data. Our method, NEUMA (Normalization by Expected Uniquely Mappable Area), is based on effective length normalization using uniquely mappable areas of gene an...

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Detalhes bibliográficos
Main Authors: Lee, Soohyun, Seo, Chae Hwa, Lim, Byungho, Yang, Jin Ok, Oh, Jeongsu, Kim, Minjin, Lee, Sooncheol, Lee, Byungwook, Kang, Changwon, Lee, Sanghyuk
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3025570/
https://ncbi.nlm.nih.gov/pubmed/21059678
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq1015
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