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Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex

BACKGROUND: Cell biologists face the need to rapidly analyse their proteins of interest in order to gain insight into their function. Often protein purification, cellular localisation and Western blot analyses can be multi-step processes, where protein is lost, activity is destroyed or effective ant...

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Autors principals: Kim, Ji Hun, Chang, Tsz M, Graham, Alison N, Choo, K HA, Kalitsis, Paul, Hudson, Damien F
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3022668/
https://ncbi.nlm.nih.gov/pubmed/21194474
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2091-11-50
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