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Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex

BACKGROUND: Cell biologists face the need to rapidly analyse their proteins of interest in order to gain insight into their function. Often protein purification, cellular localisation and Western blot analyses can be multi-step processes, where protein is lost, activity is destroyed or effective ant...

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Detalhes bibliográficos
Main Authors: Kim, Ji Hun, Chang, Tsz M, Graham, Alison N, Choo, K HA, Kalitsis, Paul, Hudson, Damien F
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3022668/
https://ncbi.nlm.nih.gov/pubmed/21194474
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2091-11-50
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