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Force Spectroscopy of Substrate Molecules En Route to the Proteasome's Active Sites

We used an atomic force microscope to study the mechanism underlying the translocation of substrate molecules inside the proteasome. Our specific experimental setup allowed us to measure interaction forces between the 20S proteasome and its substrates. The substrate (β-casein) was covalently bound e...

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Detalhes bibliográficos
Main Authors: Classen, Mirjam, Breuer, Sarah, Baumeister, Wolfgang, Guckenberger, Reinhard, Witt, Susanne
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3021671/
https://ncbi.nlm.nih.gov/pubmed/21244845
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.12.3689
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