Interlaboratory Evaluation of Different Extraction and Real-Time PCR Methods for Detection of Coxiella burnetii DNA in Serum

In the Netherlands, there is an ongoing and unparalleled outbreak of Q fever. Rapid and reliable methods to identify patients infected with Coxiella burnetii, the causative agent of Q fever, are urgently needed. We evaluated the performance of different DNA extraction methods and real-time PCR assay...

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Main Authors: Tilburg, Jeroen J. H. C., Melchers, Willem J. G., Pettersson, Annika M., Rossen, John W. A., Hermans, Mirjam H. A., van Hannen, Erik J., Nabuurs-Franssen, Marrigje H., de Vries, Maaike C., Horrevorts, Alphons M., Klaassen, Corné H. W.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2010
Fag:
Bacteriology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3020840/
https://ncbi.nlm.nih.gov/pubmed/20826645
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01006-10
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