Contamination of Commercial PCR Master Mix with DNA from Coxiella burnetii

Contamination of an in-house diagnostic real-time PCR for Q fever was traced back to a commercially obtained PCR Master Mix. It was established that this Master Mix contained DNA from Coxiella burnetii, probably as a result of the use of compounds of animal origin such as bovine serum albumin.

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Detalhes bibliográficos
Main Authors: Tilburg, Jeroen J. H. C., Nabuurs-Franssen, Marrigje H., van Hannen, Erik J., Horrevorts, Alphons M., Melchers, Willem J. G., Klaassen, Corné H. W.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Chlamydiology and Rickettsiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3008432/
https://ncbi.nlm.nih.gov/pubmed/20962145
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00464-10
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