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A Rapid, Efficient and Economical Method for Generating Leishmanial Gene Targeting Constructs

Targeted gene replacement is a powerful tool in Leishmania genetics that can be time-consuming to implement. One tedious aspect that delays progress is the multi-step construction of gene targeting vectors. To accelerate this process, we developed a streamlined method that allows the assembly of a c...

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Detalhes bibliográficos
Main Authors: Fulwiler, Audrey L., Soysa, D. Radika, Ullman, Buddy, Yates, Phillip A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3018707/
https://ncbi.nlm.nih.gov/pubmed/21055426
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molbiopara.2010.10.008
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