An isotopic assay for NADPH oxidase activity and some characteristics of the enzyme from human polymorphonuclear leukocytes.

An isotopic assay for NADPH ixodase that measures the amount of NADP formed by the 6-phosphogluconate dehydrogenase reaction has been developed. Under appropriate conditions, the amount of NADP present is directly proportional to the amount of 14CO2 released from [1-14C]6-phosphogluconic acid. Becau...

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Bibliographic Details
Published in:J Clin Invest
Main Authors: DeChatelet, L R, McPhail, L C, Mullikin, D, McCall, C E
Format: Artigo
Language:Inglês
Published: American Society for Clinical Investigation 1975
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC301807/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/235561/
https://ncbi.nlm.nih.govhttps://doi.org/10.1172/JCI107981
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