Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy

We demonstrate the measurement of two-photon excitation (TPE) spectra, used not only for fluorescence but also for photoconversion in green-to-red photoconvertible Kaede, using nonlinear Fourier-transform spectroscopy. It was found that in unphotoconverted Kaede, the TPE spectrum for photoconversion...

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Bibliografiske detaljer
Main Authors: Isobe, Keisuke, Hashimoto, Hiroshi, Suda, Akira, Kannari, Fumihiko, Kawano, Hiroyuki, Mizuno, Hideaki, Miyawaki, Atsushi, Midorikawa, Katsumi
Format: Artigo
Sprog:Inglês
Udgivet: Optical Society of America 2010
Fag:
Molecular Imaging and Probe Development
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3017996/
https://ncbi.nlm.nih.gov/pubmed/21258500
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.1.000687
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