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pH-sensitivity of the ribosomal peptidyl transfer reaction dependent on the identity of the A-site aminoacyl-tRNA

We studied the pH-dependence of ribosome catalyzed peptidyl transfer from fMet-tRNA(fMet) to the aa-tRNAs Phe-tRNA(Phe), Ala-tRNA(Ala), Gly-tRNA(Gly), Pro-tRNA(Pro), Asn-tRNA(Asn), and Ile-tRNA(Ile), selected to cover a large range of intrinsic pK(a)-values for the α-amino group of their amino acids...

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Autors principals: Johansson, Magnus, Ieong, Ka-Weng, Trobro, Stefan, Strazewski, Peter, Åqvist, Johan, Pavlov, Michael Y., Ehrenberg, Måns
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3017169/
https://ncbi.nlm.nih.gov/pubmed/21169502
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1012612107
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