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Expression, Identification and Purification of Dictyostelium Acetoacetyl-CoA Thiolase Expressed in Escherichia coli

Acetoacetyl-CoA thiolase (AT) is an enzyme that catalyses the CoA-dependent thiolytic cleavage of acetoacetyl-CoA to yield 2 molecules of acetyl-CoA, or the reverse condensation reaction. A full-length cDNA clone pBSGT-3, which has homology to known thiolases, was isolated from Dictyostelium cDNA li...

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Detalhes bibliográficos
Main Authors: Tanaka, Takeshi, Shima, Yasuyuki, Ogawa, Naoki, Nagayama, Koki, Yoshida, Takashi, Ohmachi, Tetsuo
Formato: Artigo
Idioma:Inglês
Publicado em: Ivyspring International Publisher 2010
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3014551/
https://ncbi.nlm.nih.gov/pubmed/21209787
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