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Amp-PCR: Combining a Random Unbiased Phi29-Amplification with a Specific Real-Time PCR, Performed in One Tube to Increase PCR Sensitivity

In clinical situations where a diagnostic real-time PCR assay is not sensitive enough, leading to low or falsely negative results, or where detection earlier in a disease progression would benefit the patient, an unbiased pre-amplification prior to the real-time PCR could be beneficial. In Amp-PCR,...

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Autori principali: Erlandsson, Lena, Nielsen, Lars Peter, Fomsgaard, Anders
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2010
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3013126/
https://ncbi.nlm.nih.gov/pubmed/21209824
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0015719
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