Chimeric Lactase Capable of Spontaneous and Strong Immobilization on Cellulose and Development of a Continuous-Flow System for Lactose Hydrolysis at High Temperatures

Recombinant plasmids containing fusion proteins composed of two different modules were constructed and expressed in Escherichia coli. The modules encoded the lactase LacA (LacZ) from the thermophilic bacterium Thermoanaerobacter ethanolicus and the cellulase CelD, a cellulose-binding module (CBM) fr...

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Detalhes bibliográficos
Main Authors: Velikodvorskaya, G. A., Tikhonova, T. V., Gurvits, I. D., Karyagina, A. S., Lavrova, N. V., Sergienko, O. V., Tashlitskii, V. N., Lunina, N. A., Lunin, V. G.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Biotechnology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3008238/
https://ncbi.nlm.nih.gov/pubmed/20935120
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01517-10
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