Development of a quantitative RT-PCR assay to examine the kinetics of ribosome depurination by ribosome inactivating proteins using Saccharomyces cerevisiae as a model

Ricin produced by the castor bean plant and Shiga toxins produced by pathogenic Escherichia coli (STEC) and Shigella dysenteriae are type II ribosome inactivating proteins (RIPs), containing an enzymatically active A subunit that inhibits protein synthesis by removing an adenine from the α-sarcin/ri...

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Bibliografiske detaljer
Main Authors: Pierce, Michael, Kahn, Jennifer Nielsen, Chiou, Jiachi, Tumer, Nilgun E.
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2011
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Method
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3004061/
https://ncbi.nlm.nih.gov/pubmed/21098653
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2375411
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