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Quantitative approaches to monitor protein–nucleic acid interactions using fluorescent probes

Sequence-specific recognition of nucleic acids by proteins is required for nearly every aspect of gene expression. Quantitative binding experiments are a useful tool to measure the ability of a protein to distinguish between multiple sequences. Here, we describe the use of fluorophore-labeled oligon...

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Autori principali: Pagano, John M., Clingman, Carina C., Ryder, Sean P.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cold Spring Harbor Laboratory Press 2011
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3004055/
https://ncbi.nlm.nih.gov/pubmed/21098142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2428111
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