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Quantitative approaches to monitor protein–nucleic acid interactions using fluorescent probes
Sequence-specific recognition of nucleic acids by proteins is required for nearly every aspect of gene expression. Quantitative binding experiments are a useful tool to measure the ability of a protein to distinguish between multiple sequences. Here, we describe the use of fluorophore-labeled oligon...
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| Autori principali: | , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Cold Spring Harbor Laboratory Press
2011
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3004055/ https://ncbi.nlm.nih.gov/pubmed/21098142 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2428111 |
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