Purification, crystallization and preliminary X-ray diffraction analysis of the carbohydrate-binding region of the Streptococcus gordonii adhesin GspB

The carbohydrate-binding region of the bacterial adhesin GspB from Strepto­coccus gordonii strain M99 (GspB(BR)) was expressed in Escherichia coli and purified using affinity and size-exclusion chromatography. Separate sparse-matrix screening of GspB(BR) buffered in either 20 mM Tris pH 7.4 or 20 mM...

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Autores principales: Pyburn, Tasia M., Yankovskaya, Victoria, Bensing, Barbara A., Cecchini, Gary, Sullam, Paul M., Iverson, T. M.
Formato: Artigo
Lenguaje:Inglês
Publicado: International Union of Crystallography 2010
Materias:
Crystallization Communications
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3001660/
https://ncbi.nlm.nih.gov/pubmed/21045307
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110036535
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