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Use of the GTPγS ([(35)S]GTPγS and Eu-GTPγS) binding assay for analysis of ligand potency and efficacy at G protein-coupled receptors
In this review I consider assays for G protein-coupled receptor (GPCR) activity based on the binding of labelled analogues of GTPγS ([(35)S]GTPγS or Eu-GTPγS) to G proteins in tissues (GTPγS binding assays). Such assays provide convenient measures of GPCR activity close to the receptor in the signal...
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| Autor principal: | |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Blackwell Science Inc
2010
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3000650/ https://ncbi.nlm.nih.gov/pubmed/20662841 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1476-5381.2010.00963.x |
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