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High-level expression of sperm whale myoglobin in Escherichia coli.
Sperm whale myoglobin was expressed in Escherichia coli from a totally synthetic gene inserted in the expression vector pUC19. The gene was constructed as 23 overlapping oligonucleotides encoding both strands of the DNA. Gene synthesis provides several advantages over traditional eukaryotic gene-clo...
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| 出版年: | Proc Natl Acad Sci U S A |
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| 主要な著者: | , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
National Academy of Sciences
1987
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC299671/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3321062/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.24.8961 |
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