High-level expression of sperm whale myoglobin in Escherichia coli.
Sperm whale myoglobin was expressed in Escherichia coli from a totally synthetic gene inserted in the expression vector pUC19. The gene was constructed as 23 overlapping oligonucleotides encoding both strands of the DNA. Gene synthesis provides several advantages over traditional eukaryotic gene-clo...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1987
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC299671/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3321062/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.24.8961 |
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