In vitro synthesis of vaccinia virus late mRNA containing a 5' poly(A) leader sequence.

We have shown that an extract made from HeLa cells harvested 6 hr after infection with vaccinia virus can transcribe a duplex DNA template containing a late viral gene. S1 nuclease analyses using genomic and synthetic probes indicated that the 5' ends of RNA synthesized in vitro are similar to...

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Bibliographic Details
Main Authors: Wright, C F, Moss, B
Format: Artigo
Language:Inglês
Published: 1987
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC299655/
https://ncbi.nlm.nih.gov/pubmed/2827158
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