In vitro synthesis of vaccinia virus late mRNA containing a 5' poly(A) leader sequence.
We have shown that an extract made from HeLa cells harvested 6 hr after infection with vaccinia virus can transcribe a duplex DNA template containing a late viral gene. S1 nuclease analyses using genomic and synthetic probes indicated that the 5' ends of RNA synthesized in vitro are similar to those...
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| Published in: | Proc Natl Acad Sci U S A |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
1987
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC299655/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2827158/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.24.8883 |
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