RiboSys, a high-resolution, quantitative approach to measure the in vivo kinetics of pre-mRNA splicing and 3′-end processing in Saccharomyces cerevisiae

We describe methods for obtaining a quantitative description of RNA processing at high resolution in budding yeast. As a model gene expression system, we constructed tetON (for induction studies) and tetOFF (for repression, derepression, and RNA degradation studies) yeast strains with a series of re...

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Detalhes bibliográficos
Main Authors: Alexander, Ross D., Barrass, J. David, Dichtl, Beatriz, Kos, Martin, Obtulowicz, Tomasz, Robert, Marie-Cecile, Koper, Michal, Karkusiewicz, Iwona, Mariconti, Luisa, Tollervey, David, Dichtl, Bernhard, Kufel, Joanna, Bertrand, Edouard, Beggs, Jean D.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2010
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2995417/
https://ncbi.nlm.nih.gov/pubmed/20974745
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2162610
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