Optimization of a serum-free culture medium for mouse embryonic stem cells using design of experiments (DoE) methodology

The in vitro culture behaviour of embryonic stem cells (ESC) is strongly influenced by the culture conditions. Current culture media for expansion of ESC contain some undefined substances. Considering potential clinical translation work with such cells, the use of defined media is desirable. We have...

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Bibliografiske detaljer
Main Authors: Knöspel, Fanny, Schindler, Rudolf K., Lübberstedt, Marc, Petzolt, Stephanie, Gerlach, Jörg C., Zeilinger, Katrin
Format: Artigo
Sprog:Inglês
Udgivet: Springer Netherlands 2010
Fag:
Original Research
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2995139/
https://ncbi.nlm.nih.gov/pubmed/20859764
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-010-9307-8
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