Optimization of a serum-free culture medium for mouse embryonic stem cells using design of experiments (DoE) methodology

The in vitro culture behaviour of embryonic stem cells (ESC) is strongly influenced by the culture conditions. Current culture media for expansion of ESC contain some undefined substances. Considering potential clinical translation work with such cells, the use of defined media is desirable. We have...

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Autori principali: Knöspel, Fanny, Schindler, Rudolf K., Lübberstedt, Marc, Petzolt, Stephanie, Gerlach, Jörg C., Zeilinger, Katrin
Natura: Artigo
Lingua:Inglês
Pubblicazione: Springer Netherlands 2010
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Original Research
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2995139/
https://ncbi.nlm.nih.gov/pubmed/20859764
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-010-9307-8
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