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Differentiation of Mouse Embryonic Stem Cells into Endoderm without Embryoid Body Formation

Pluripotent embryonic stem cells hold a great promise as an unlimited source of tissue for treatment of chronic diseases such as Type 1 diabetes. Herein, we describe a protocol using all-trans-retinoic acid, basic fibroblast growth factor and dibutyryl cAMP (DBcAMP) in the absence of embryoid body f...

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Detalhes bibliográficos
Main Authors: Kim, Peter T. W., Hoffman, Brad G., Plesner, Annette, Helgason, Cheryl D., Verchere, C. Bruce, Chung, Stephen W., Warnock, Garth L., Mui, Alice L. F., Ong, Christopher J.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2010
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2994751/
https://ncbi.nlm.nih.gov/pubmed/21152387
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0014146
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