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Identification of methotrexate transport deficiency in mammalian cells using fluoresceinated methotrexate and flow cytometry.

We have studied the frequency of transport mutations in methotrexate-resistant Chinese hamster ovary cells using a rapid-flow cytometric technique. After saturating cells with fluoresceinated methotrexate, we examined the ability of hydrophilic and lipophilic antifolates to displace fluoresceinated...

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Detalhes bibliográficos
Main Authors: Assaraf, Y G, Schimke, R T
Formato: Artigo
Idioma:Inglês
Publicado em: 1987
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC299248/
https://ncbi.nlm.nih.gov/pubmed/3478688
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