Native Electrospray and Electron-Capture Dissociation in FTICR Mass Spectrometry Provide Top-Down Sequencing of a Protein Component in an Intact Protein Assembly

The intact yeast alcohol dehydrogenase (ADH) tetramer of 147 kDa was introduced into a FTICR mass spectrometer by native electrospray. Electron capture dissociation of the entire 23+ to 27+ charge state distribution produced the expected charge-reduced ions and, more unexpectedly, 39 c-type peptide...

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Hlavní autoři: Zhang, Hao, Cui, Weidong, Wen, Jianzhong, Blankenship, Robert E., Gross, Michael L.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2010
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2991543/
https://ncbi.nlm.nih.gov/pubmed/20843701
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jasms.2010.08.006
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